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. Author manuscript; available in PMC: 2013 Mar 20.
Published in final edited form as: J Immunol. 2008 Jul 1;181(1):574–585. doi: 10.4049/jimmunol.181.1.574

FIGURE 3.

FIGURE 3

VRP induce the production of large molecular mass IgA Abs in the DLN. Groups of BALB/c mice were immunized in the rear footpad with HA-VRP (1 × 105 IU), I-Flu alone (10 μg), or I-Flu (10 μg) plus GFP-VRP (1 × 105 IU) at weeks 0 and 4. At day 3 after boost, DLN and contralateral lymph node (CLN) culture supernatants were evaluated for IgA Abs by nonreducing Western blot analysis (A). Day 3 DLN supernatants were then mixed with influenza virus to form virus-Ab complexes and complexes were purified via ultracentrifugation before nonreducing Western blot analysis for IgA (B). Groups of BALB/c mice were immunized in the rear footpads with OVA alone (10 μg), OVA (10 μg) plus null VRP (1 × 105 IU), or OVA (10 μg) plus CpG DNA (1 μg) at weeks 0 and 4 and DLNs were harvested at day 3 and DLN PBS homogenates were evaluated for IgA Abs by nonreducing Western blot analysis as in A (C). The gel lanes in A have been rearranged from the original order for esthetic purposes; however, the data are all from the same gel from a single experiment.