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. Author manuscript; available in PMC: 2013 Mar 20.
Published in final edited form as: J Immunol. 2008 Jul 1;181(1):574–585. doi: 10.4049/jimmunol.181.1.574

FIGURE 5.

FIGURE 5

Characterization of VRP DLN cells. Groups of BALB/c mice were immunized in the rear footpads at weeks 0 and 4 with I-Flu alone (1 μg), I-Flu (1 μg) coinoculated with null VRP (1 × 105 IU), or null VRP alone (1 × 105 IU). At days 0, 1, and 3 after boost, DLNs were harvested and weighed on an analytical balance and single-cell suspensions were prepared by collagenase digestion. The mass of each lymph node was determined (A) and total number of cells was determined by trypan blue exclusion (B). The number of B cells (C), T cells (D), CD11b− DCs (E), and CD11b+ DCs (F) was evaluated by flow cytometry. Values are presented as geometric mean ± SEM. *, p < 0.05; **, p < 0.01; and ***, p < 0.001 compared with I-Flu alone, as determined by ANOVA.