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. Author manuscript; available in PMC: 2013 Mar 20.
Published in final edited form as: J Immunol. 2008 Jul 1;181(1):574–585. doi: 10.4049/jimmunol.181.1.574

FIGURE 6.

FIGURE 6

VRP induce the expression of the mucosal homing receptor on DLN B cells. Groups of animals were immunized in the rear footpad at weeks 0 and 4 with OVA alone (10 μg) or OVA (10 μg) coinoculated with null VRP (1 × 105 IU). At days 1 and 3 after boost, DLN cells were stained with Abs directed against CD19, B220, and the α4β7 integrin and analyzed by flow cytometry. As a positive control, α4β7 integrin expression was examined on MLN cells (A). α4β7 integrin expression in both B220high and B220low DLN cells is shown in B. The histograms in A and B are all on the same scale, except the expanded inset in B. To confirm the up-regulation of α4β7 integrin expression specifically in the B220 low population following delivery of a viral Ag, α4β7 integrin expression was analyzed on DLN cells at days 0, 1, and 3 after boost following delivery of I-Flu alone (1 μg), I-Flu (1 μg) plus null VRP (1 × 105 IU), or null VRP alone (1 × 105 IU) (C). Values are presented as geometric mean ± SEM. *, p < 0.001 compared with I-Flu alone, as determined by ANOVA.