Table 2.
Flow cytometric analysis of Annexin V:FITC assay in CEMss cells treated with IC50 concentration of ZC-B11 for 6, 12, 24, and 48 hours. Untreated cells serve as negative control. Data were represented as means ± SD of at least three independent experiments.
| Number of cells (%) ± SD | |||||
|---|---|---|---|---|---|
| Untreated | 6 | 12 | 24 | 48 | |
| Viable | 97.30 ± 0.42 | 96.80 ± 0.35 | 95.20 ± 0.57 | 86.70 ± 1.06 | 83.20 ± 0.78 |
| Early apoptosis | 2.20 ± 0.21 | 3.00 ± 0.14 | 3.80 ± 0.42 | 12.80 ± 1.13* | 15.60 ± 1.20* |
| Late apoptosis/secondary necrosis |
0.50 ± 0.21 | 0.30 ± 0.49 | 0.60 ± 0.07 | 1.10 ± 0.21 | 1.20 ± 0.43 |
*indicates a significant difference from the control (P < 0.05).