Table 3. TCA cycle and related components identified for genomic bins r9c1-5 and r9c7.

#	Enzyme	EC	1	2	3	4	5	7
1	Citrate synthase	2.3.3.1	—	—	G	—	P	P
1	ATP citrate lyase	2.3.3.8	P	P	P	—	—	—
1	Citrate lyase	4.1.3.6	P	G	G	G	G	—
2	Aconitate hydratase	4.2.1.3	P	G	P	—	G	G
3	Isocitrate dehydrogenase	1.1.1.41/42	P	P	P	P	G	—
4a	2-oxoglutarate dehydrogenase E1	1.2.4.2	—	—	—	G	—	—
4b	2-oxoglutarate dehydrogenase E2	2.3.1.61	—	—	—	G	G	—
4c	Dihydrolipoamide dehydrogenase	1.8.1.4	P	—	—	G	G	—
4	2-oxoglutarate synthase	1.2.7.3	P	P	P	G	G	—
5	Succinyl-CoA synthetase	6.2.1.4/5	P	P	P	G	G	—
6	Succinate/fumarate reductase	1.3.5.1a	P	P	—	P	P	P
7	Fumarate hydratase	4.2.1.2	P	G	G	—	G	—
8	Malate dehydrogenase (NAD-dependent)	1.1.1.37	—	P	G	P	G	P
8	Malate dehydrogenase (quinone)	1.1.5.4	—	—	G	G	—	—
	Related: pyruvate/PEP
	PEP synthetase	2.7.9.2	P	G	G	G	G	—
	PEP carboxylase (GTP)	4.1.1.32	P	—	—	—	—	—
	PEP carboxylase (ATP)	4.1.1.49	—	—	—	—	—	—
	PEP carboxylase	4.1.1.31	G	—	—	—	P	—
	Pyruvate/oxaloacetate carboxylase	6.4.1.1b	P	P	P	G	G	—
	Pyruvate ferredoxin oxidoreductase	1.2.7.1	P	G	P	P	—	—
a	Pyruvate dehydrogenase E1	1.2.4.1	P	G	—	G	G	—
b	Pyruvate dehydrogenase E2	2.3.1.12	P	—	—	—	—	—
c	(4c above)
	Related: acetate↔acetyl-CoA
	Acetyl-CoA hydrolase/transferase	3.1.2.1	P	—	—	—	G	—
	Acetyl-CoA synthetase	6.2.1.1	P	G	G	—	—	—
a	Acetate kinase (acetyl-P↔acetate)	2.7.2.1	P	G	G	G	G	—
a	Acylphophatase (acetyl-P→acetate)	3.6.1.7		G	—	G	G	—
b	Phosphate acetyltransferase	2.3.1.8	P	G	G	G	G	—

Abbeviations: #, order of TCA cycle reactions in the oxidative direction; EC, enzyme commission number; G, genes only identified; P, proteins also detected.

Reactions for enzymes not previously defined here: pyruvate carboxylase (pyruvate→oxaloacetate); pyruvate ferredoxin oxidoreductase (i.e., pyruvate synthase; pyruvate↔acetyl-CoA); pyruvate dehydrogenase and dihydrolipoamide dehydrogenase (pyruvate↔acetyl-CoA).

^aSuccinate/fumarate reductase equates to EC:1.3.5.1/EC:1.3.99.1.

^bpyruvate carboxylase equates to EC:6.4.1.1/EC:4.1.1.3.