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. 2013 Jan 17;2(3):277–282. doi: 10.1242/bio.20133202

Fig. 3. Reciprocal effects on expression levels of Limk2 and PlexinA1 knockdown.

Fig. 3.

(A) QPCR analysis of rat E16 MGE cells transfected with Limk2-siRNA, PlexinA1-siRNA or control constructs; PCR products are shown on the left. (B,C) Immunoblots of MGE cells transfected with Limk2-siRNA and PlexinA1-siRNA showed reduced levels of Limk2 and PlexinA1, but not neuropilins compared to controls. (D,E) Migration of MGE cells in an E14 mouse embryo electroporated in utero with control-siRNA (D) or PlexinA1-siRNA (E). (F) Quantification of electroporated cells (dsRed+) entering the striatum. (D′,E′) Schematic representation of the migratory routes adopted by MGE cells electroporated with control-siRNA (D′) and PlexinA1-siRNA (E′). Scale bar: E, 200 µm. Str, striatum; WB, Western blot. **P<0.001.