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. 2013 Jan 17;2(3):314–323. doi: 10.1242/bio.20133327

Fig. 3. Acentriolar spindle poles do not recruit centrosomal components.

Fig. 3.

Immunofluorescence analysis of control cells Jupiter (upper panel) and acentriolar cells of line 131 (lower panel) for recruitment of PCM proteins. In all cases PCM proteins are strongly recruited at the centrosomes (arrows) in wild-type cells. On the contrary these proteins are dispersed in the cytoplasm in acentriolar cells. Blue: DNA, Green: α-tubulin, White/Red: centrosomal protein. (A,D) Centrosomin staining. (B,E) γ-tubulin 23C. In acentriolar cells the centrosomal staining disappears although a faint spindle staining is conserved. (C,F) D-PLP staining. In (C) (control cells), arrows indicate centriolar structures at the mitotic poles and arrowhead an interphasic centriolar structure. In (F) D-PLP positive granules are found scattered in the cytoplasm of acentriolar cells. Scale bar: 10 µm.