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. 2013 Jan 30;2(3):351–361. doi: 10.1242/bio.20133830

Fig. 3. Substrate elasticity independent formation of costameres.

Fig. 3.

GFP-α-actinin transfected cells (A) were grown for 3 days on 15 kPa, 30 kPa and 90 kPa substrates. Live cell images were segmented using image processing (B). Scale bar: 10 µm. Mean α-actinin cluster size (C), relative coverage of cells with α-actinin clusters (D) and the total number of α-actinin clusters per cell (E) were determined. All results are indicated with their respective s.d.; n = 16 cells for 15 kPa, 20 cells for 30 kPa and 13 cells for 90 kPa.