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. 2013 Feb;3(2):120158. doi: 10.1098/rsob.120158

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© 2013 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 1. — Fluorescence labelling of yeast strains. (a) Wild-type yeast transformed with plasmids expressing Venus (yellow fluorescent protein), Sapphire (blue fluorescent protein), mCherry (red fluorescent protein) or CFP (cyan fluorescent protein); visualized under bright field (BF) or ultraviolet (UV) light. (b) Schematic view of the experimental design developed for high-throughput screens: yeast strains expressing heterologous drug-resistant Plasmodium falciparum DHFR (^PfRdhfr), Schistosoma mansoni DHFR (^SmDHFR), human DHFR (^HsDHFR) or P. falciparum DHFR (^PfDHFR) growing in the presence of candidate anti-parasitic drugs. (c) Pictures of fluorescently labelled yeast strains (expressing the indicated heterologous DHFRs) grown in competition in the presence or absence of the anti-malarial pyrimethamine.