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. 2013 Feb;3(2):120158. doi: 10.1098/rsob.120158

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© 2013 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 2. — Relative fluorescence measure detected using a BMG Optima plate reader at 580 nm (excitation)/612 nm (emission) (Cherry), 405 nm (excitation)/510 nm (emission) (Sapphire), 500 nm (excitation)/540 nm (emission) (Venus) and 440 nm (excitation)/490 nm (emission) (CFP) of pooled yeast strains grown for 24 h in the presence of 0 to 500 μM pyrimethamine and 0 or 5 μg ml⁻¹ of doxycycline. This plate reader has a limited dynamic range, and a higher-resolution instrument was used for the high-throughput screens. Plasmodium falciparum DHFR (^PfRdhfr) labelled with Venus, S. mansoni DHFR (^SmDHFR) labelled with CFP, human DHFR (^HsDHFR) labelled with mCherry and P. falciparum DHFR (^PfDHFR) labelled with Venus.