Figure 3.

Example of a high-throughput screening result. Relative fluorescence measure detected using a BMG Polarstar plate reader at 580 nm (excitation)/612 nm (emission) (Cherry), 405 nm (excitation)/ 510 nm (emission) (Sapphire) and 500 nm (excitation)/540 nm (emission) (Venus) of pooled yeast strains grown in the presence of 5 μg ml⁻¹ of doxycycline AND 10 μM pyrimethamine, 10 μM of test compound or no drug. Plasmodium vivax DHFR labelled with Sapphire (blue squares), human DHFR labelled with mCherry (red squares) and drug-resistant P. falciparum DHFR (PfRdhfr) labelled with Venus (yellow squares).