Figure 4.

Network of connections between kinetoplastid targets and our hits. Overview of the compounds from the Maybridge hitfinder library identified as specific inhibitors of the following parasite (Trypanosoma brucei, Tb; T. cruzi, Tc; Leishmania major, Lm) targets: dihydrofolate reductase (DHFR), blue; N-myristoyltransferase (NMT), red; phosphoglycerate kinase (PGK), green. Small nodes represent hits; yellow nodes represent compounds tested in T. brucei cultures. Diamond nodes represent compounds active in Trypanosoma at 10 µM. Large diamonds represent compounds active in T. brucei cultures at 1 µM or less. The thickness of the edges (lines connecting the targets and compounds) represents the strength of the inhibition (thicker lines indicate stronger inhibition of growth of the yeast expressing the indicated parasite target by the connecting compound).