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. 2013 Feb;3(2):120175. doi: 10.1098/rsob.120175

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© 2013 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 6. — PduO is essential for B₁₂ salvage and assimilation. (a) Predicted steps in late-stage AdoCbl biosynthesis and salvage in M. tuberculosis. Cobinamide is provided in vitro as a dicyanide salt, (CN)₂Cbi. (b) The ΔmetH pduO::Tn mutant cannot use CNCbl or (CN)₂Cbi for growth in 0.1% propionate-containing 3NP. Data are from a representative experiment performed in duplicate.