Figure 4.

Recombinant RANKL and RANK overexpression activate NF-κB in SCG neurons. (a) NF-κB reporter signal in P0 SCG neurons incubated for 4 and 24 h in medium containing 10 ng ml⁻¹ NGF alone (unfilled bars) and 10 ng ml^–1 NGF plus 10 ng ml^–1 RANKL (filled bars). (b) NF-κB reporter signal in P0 SCG neurons transfected with either a RANK (filled bars) expression plasmid or a control (unfilled bars) empty plasmid and incubated for 4 and 24 h in medium containing 10 ng ml^–1 NGF. The data are normalized to the respective control signal. Mean ± s.e.m. are shown, **p < 0.001, statistical comparison with the respective controls. The non-normalized datasets of these experiments are provided in electronic supplementary material, spreadsheet S4.