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. 2013 Jan;3(1):120163. doi: 10.1098/rsob.120163

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© 2013 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 2. — CD and visible absorption spectra of Sco^Sl and the H176A mutant. (a) Far-UV CD spectra of apo-oxidized (disulphide), apo-reduced (free-thiol) and Cu(II)-loaded Sco^Sl. (b) Visible absorbance spectra of Cu(II)-loaded Sco^Sl and the H176A mutant. All spectra were recorded at 20°C, with protein concentrations ranging between 20 and 100 μM.