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. 2013 Jan;3(1):120163. doi: 10.1098/rsob.120163

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© 2013 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 3. — Cu(II) binding to reduced apo-Sco^Sl. (a) Changes in the UV–vis spectrum (pH 7.5, 20°C) of reduced apo-Sco^Sl (110 μM) upon stepwise addition of 10 μM Cu(II)SO₄. Inset: the change in absorbance at 362 nm plotted as a function of [Cu(II)]/[Sco^Sl]. (b) Visible CD spectra of Cu(II)-loaded and apo-reduced Sco^Sl and the H176A mutant. (c) Inset: changes in the Trp emission spectrum (pH 7.5, 20°C) of reduced apo-Sco^Sl (5 μM) upon titration with 0.5 μM of Cu(II)SO₄ with the emission at 330 nm plotted as a function of [Cu(II)]/[Sco^Sl]. The arrow indicates the direction of the emission change. The stoichiometry of the reaction in both (a) and (c) is indicated by the intersection of the two lines.