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Stopped-flow kinetics of Cu(II) binding to the H176A mutant. (a) Global fitting of time courses at wavelengths from 420 to 320 nm to a sequential model a > b > c. (b) First-order rate constants for the ‘fast’ (k1_H176A; filled squares) and ‘slow’ (k2_H176A; open triangles) phases of Cu(II) binding to the H176A as a function of Cu(II) concentration. The dashed line indicates the [Cu(II)] independence for k2_H176A.
