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. 2013 Apr 20;18(12):1385–1399. doi: 10.1089/ars.2012.4569

FIG. 4.

FIG. 4.

Social isolation induces a late increase of oxidative stress in the rat adrenal gland. (A–F) Representative images of DAB immunohistochemistry for 8OhdG in control (A) and isolated rats for a period of 2W (B), 4W (D), and 7W (E) of social isolation, (n=6 for each group in each time point). (C, F) are magnified views of the dotted box in (B, E), respectively. (G–L) Representative images of DAB immunohistochemistry for nitrotyrosine in control (G) and isolated rats for a period of 2W (H), 4W (J), and 7W (K) of social isolation (n=6 for each group in each time point). (I, L) are magnified views of the dotted box in (H, K), respectively. (M) Quantification of 8OhdG-immunoreactive cells in the adrenal glands of GRP and ISO rats after a period of 2W, 4W, and 7W (n=6 for each group in each time point). Statistical analysis: Two-way analysis of variance followed by Tukey post hoc test. Fr(1,30)=105.918 p<0.001; Ft(2,30)=98.500 p<0.001; Ftxr(2,30)=99.244 p<0.001 ***p<0.001, not significant; rearing within 2W p=0.996, rearing within 4W p=0.701. (N) Quantification of nitrotyrosine-immunoreactive cells in the adrenal glands of GRP and ISO rats after a period of 2W, 4W, and 7W (n=6 for each group in each time point). Statistical analysis: Two-way analysis of variance followed by Tukey post hoc test. Fr(1,30)=149.028 p<0.001; Ft(2,30)=132.718 p<0.001; Ftxr(2,30)=137.412 p<0.001; ***p<0.001. (O) Real-time PCR for NOX2 mRNA expression (fold change) in the adrenal glands of GRP and ISO rats after a period of 2W, 4W, and 7W (n=6 for each group in each time point). Statistical analysis: Two-way analysis of variance followed by Tukey post hoc test. Fr(1,30)=81.995 p<0.001; Ft(2,30)=81.996 p<0.001; Ftxr(2,30)=81.995p<0.001; ***p<0.001. Scale bar: 58 μM.