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. 2013 Jan 1;126(1):163–175. doi: 10.1242/jcs.111203

Fig. 6.

Fig. 6.

Co-depletion of Poc1A and Poc1B leads to loss of centrosome proteins. HeLa cells were transfected with siRNAs against luciferase (siGL2) or Poc1A+Poc1B, as indicated, and fixed after 72 hours. Cells were stained with γ-tubulin antibodies alone (A, red), γ-tubulin (red) and FOP antibodies (green; C,G), Cep135 antibodies (green) (D), C-Nap1 (red) and Sas-6 (green; E), γ-tubulin (red) and Cep164 (green; H). (B) The percentage of interphase cells with 2 strong, 1 weak and 1 strong, 1 alone or 0 γ-tubulin-stained spots. Data are means ± s.d. from two independent experiments (n = 207 for siGL2 and n = 222 for siPoc1A+siPoc1B). (F) The percentage of interphase cells with 2 strong, 1 weak and 1 strong, 1 alone or 0 C-Nap1-positive centrosomes were scored with respect to the number of Sas-6 dots seen in the same cells. Data are from a single experiment (n = 100 for siGL2 and n = 110 for siPoc1A+siPoc1B). Merged images include DNA staining with Hoechst 33258 (blue). Magnified views of centrosomes, when visible, are shown. Scale bars: 6 µm (A,C,D,E,H); 11 µm (G).