Fig. 5.

DCC mutants had reduced axon guidance induced by netrin. (A) Examples of axons growing and the netrin-coated beads (white circle). Scale bar: 20 µm. (B) Compared with WT, the numbers of axons growing towards netrin-coated beads in N329P, Q361S and Δlinker2-3 DCC mutants were significantly decreased. In addition, the length of axons was reduced in neurons microinjected with mutant DCC. Beads coated with PBS or BSA did not attract axons. Data are means±s.e.m. (n = 6). **P<0.01. (C) In COS-7 cells, netrin-binding assays showed binding ability of netrin to non-transfected cells, vector only, WT, N329P, Q361S and Δlinker2-3 DCC mutants at the cell surface. Scale bar: 20 µm.