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. 2013 Jan 1;126(1):221–233. doi: 10.1242/jcs.113415

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© 2013. Published by The Company of Biologists Ltd

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Fig. 8. — Schematic diagram of signaling events. (A) A randomly moving vegetative cell. PtdIns(3,4,5)P₃ [PI(3,4,5)P3], PtdIns(4,5)P₂ [PI(4,5)P2] and signaling proteins are color coded. Pseudopods are enriched with PtdIns(3,4,5)P₃ (dark blue), PI3K, LimE, dynacortin, coronin and Ras activity (maroon signaling and cytoskeletal components). PtdIns(4,5)P₂ (orange), cortexillin-1, myosin II and PTEN are localized (light blue) to the membrane areas between the pseudopods. (B) Upon uniform chemoattractant stimulation, PtdIns(4,5)P₂ levels decrease and cortexillin-1, myosin II and PTEN delocalize from the membrane and are enriched in the cytoplasm (light blue). In contrast, the other components localize uniformly across the membrane periphery as PtdIns(3,4,5)P₃ levels increase. (C) A vegetative cell migrating in a FA gradient. Pseudopods are biased towards the FA source and enriched with PtdIns(3,4,5)P₃ and maroon signaling and cytoskeletal components. More PtdIns(4,5)P₂, PTEN, cortexillin-1, myosin II and PTEN were found at the plasma membrane away from the FA source. (D) Similar localization was previously observed in polarized cells exposed to a cAMP gradient. PtdIns(3,4,5)P₃ localization is based on PH_Crac–GFP, while PtdIns(4,5)P₂ distribution is based on the localization of PTEN–GFP.