Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Jan 1;126(1):312–326. doi: 10.1242/jcs.116244

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013. Published by The Company of Biologists Ltd

PMC Copyright notice

Fig. 2. — Villin regulates directionally persistent cell migration in isolated cells. (A) Time-lapse images of MDCK Tet-Off VIL/NULL cells and cells expressing VIL/WT or VIL/Δ21–67/112–119 were collected over a period of 120 min. The elliptical factor was calculated as a measure of cell polarization. Values are means ± s.e.m. (n = 15). (B) The path of migration of individual VIL/NULL, VIL/WT or VIL/Δ21–67/112–119 cells was tracked using the cell track function in Metamorph software. The track of individual cells were consolidated and re-plotted from the origin. Cell migration directionality was quantified by measuring the average angle between velocity vectors as a function of distance along the trajectory L, the average tortuosity as a function of time, and the mean and square displacement as a function of time. All data were scored from 30 individual cells. The error bars indicate 95% confidence intervals.

Inline graphic — Villin regulates directionally persistent cell migration in isolated cells. (A) Time-lapse images of MDCK Tet-Off VIL/NULL cells and cells expressing VIL/WT or VIL/Δ21–67/112–119 were collected over a period of 120 min. The elliptical factor was calculated as a measure of cell polarization. Values are means ± s.e.m. (n = 15). (B) The path of migration of individual VIL/NULL, VIL/WT or VIL/Δ21–67/112–119 cells was tracked using the cell track function in Metamorph software. The track of individual cells were consolidated and re-plotted from the origin. Cell migration directionality was quantified by measuring the average angle between velocity vectors as a function of distance along the trajectory L, the average tortuosity as a function of time, and the mean and square displacement as a function of time. All data were scored from 30 individual cells. The error bars indicate 95% confidence intervals.