Figure 1.

Cig1 is required for growth in heme at physiological pH. Growth curves of iron-starved cells in low-iron medium (LIM; A); LIM plus 10 µM FeCl₃ (B); LIM plus 10 µM ferrioxamine (C); LIM plus 10 µM heme, pH 7.2 (D); and LIM plus 10 µM heme, pH 5.6 (E). Growth was monitored by measuring the optical density at 600 nm. F, Expression of CIG1 in wild-type (WT) cells grown in LIM, with or without 10 µM heme, at pH 5.6 or 7.2. All experiments were repeated 3 times, and the data are plotted as average ± SD. Statistical analysis was performed using the Student t test. *P < .05.