Table 3.
Gene bank accession numbers and primer sequences of the genes investigated by real-time RT-PCR.
| Gene name (abbreviation used) | Gene bank accession number | Primer sequences (5′ → 3′) for = forward; rev = reverse; |
|---|---|---|
| Chemokine (C-C motif) ligand 2 (Ccl2) (MCP1) | NM_031530 | for: GTGCGACCCCAATAAGGAA |
| rev: TGAGGTGGTTGTGGAAAAGA | ||
| Claudin 3 (Cldn3) | NM_031700 | for: TATCCTACTGGCAGCCTTCG |
| rev: GTTCCCATCTCTCGCTTCTG | ||
| Copper/zinc superoxide dismutase (SOD1) | NM_017050 | for: CCACTGCAGGACCTCATTTT |
| rev: CACCTTTGCCCAAGTCATCT | ||
| C-reactive protein (CRP) | NM_017096 | for: GTCTCTATGCCCACGCTGAT |
| rev: CCGTCAAGCCAAAGCTCTAC | ||
| Glutathione S-transferase K1 (GSTK1) | NM_181371 | for: GAGCATGGAGCAACCAGAGAT |
| rev: AGCTTGCTCTTCACCAGTTCG | ||
| Glutathione S-transferase P1 (GSTP1) | NM_012577 | for: GAGGCAAAGCTTTCATTGTGG |
| rev: GTTGATGGGACGGTTCAAATG | ||
| Glutathione S-transferase T2 (GSTT2) | NM_012796 | for: GAGGAAAAGGTGGAACGGAAC |
| rev: CGCCCCTCAAACAGATTACAG | ||
| Glutathione peroxidase 2 (GPx2) | NM_183402 | for: GTGTGATGTCAATGGGCAGAA |
| rev: ACGTTTGATGTCAGGCTCGAT | ||
| Heme oxygenase 1 (HO1) | NM_012580 | for: AGGCACTGCTGACAGAGGAAC |
| rev: AGCGGTGTCTGGGATGAACTA | ||
| Hypoxanthine phosphoribosyltransferase 1 (Hprt1) | NM_012583 | for: GCAGACTTTGCTTTCCTTGG |
| rev: TCCACTTTCGCTGATGACAC | ||
| Interleukin 1 beta (IL-1β) | NM_031512 | for: CTGTGACTCGTGGGATGATG |
| rev: GGGATTTTGTCGTTGCTTGT | ||
| Interleukin 10 (IL-10) | NM_012854 | for: CTGGAGTGAAGACCAGCAAAGG |
| rev: GGAGAAATCGATGACAGCGTCG | ||
| Kelch-like ECH-associated protein1 (Keap1) | NM_057152 | for: GTGGCGGATGATTACACCAAT |
| rev: GAAAAGTGTGGCCATCGTAGC | ||
| NAD(P)H dehydrogenase [quinone] 1 (NQO1) | NM_017000 | for: CGCAGAGAGGACATCATTCA |
| rev: CGCCAGAGATGACTCAACAG | ||
| Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) | NM_031789 | for: CCAAGGAGCAATTCAACGAAG |
| rev: CTCTTGGGAACAAGGAACACG | ||
| Nuclear factor kappa B (NFκB) | L26267 | for: CTTCTCGGAGTCCCTCACTG |
| rev: CCAATAGCAGCTGGAAAAGC | ||
| Prostaglandin-endoperoxide synthase 2 (Ptgs2) (COX2) | NM_017232 | for: GCTGTACAAGCAGTGGCAAA |
| rev: CCCCAAAGACAGCATCTGGA | ||
| Ribosomal protein L13A (Rpl13a) | NM_173340 | for: CCCTCCACCCTATGACAAGA |
| rev: CCTTTTCCTTCCGTTTCTCC | ||
| Tumor necrosis factor alpha (TNFα) | NM_012675 | for: GCCAATGGCATGGATCTCAAAG |
| rev: AAATCGGCTGACGGTGTGGG | ||
| Vascular cell adhesion molecule 1 (VCAM 1) | NM_012889 | for: TGACATCTCCCCTGGATCTC |
| rev: CTCCAGTTTCCTTCGCTGAC | ||
| β-actin | NM_031144 | for: ATCGTGCGTGACATTAAAGAGAAG |
| rev: GGACAGTGAGGCCAGGATAGAG |