Fig. 6.

Combined genetic targeting of HDAC6 and HDAC9 does not substantially improve the suppressive function of Tregs in vivo. (A) Homeostatic proliferation assays showed improved suppression of effector T cells by Tregs with either individual deficiency in either HDAC6 or HDAC9, but no added benefit from combined deletion. (B) Preservation of Foxp3 was not changed by combined knockout of HDAC6 and HDAC9. (C) Ki67 abundance was increased in HDAC6−/− HDAC9−/− double knockout Treg compared to that in single knockout and WT cells. Data are from three to four mice per group (N=18). The gating strategy used was similar to that in Fig. 5B. *P < 0.05, **P < 0.01, and ***P <0.001.