Fig. 1.
Nemo's kinase activity is required for Eya-Nemo synergy during eye induction. Heterozygosity for nmo reduces the frequency of Dpp-Gal4>UAS-eya-mediated ectopic eye formation, while coexpression of UAS-Nmo increases both the frequency and penetrance of ectopic eyes. A Kinase-dead Nmo transgene (NmoK69M) fails to increase and slightly suppresses ectopic eye frequency when coexpressed with Eya. Penetrance reflects whether ectopic eye tissue was observed under one or both antennae (1 Ectopic eye, dark gray bar or 2 Ectopic eyes, black bar) and frequency refers to a binary scoring system for presence/absence of ectopic eye tissue.