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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Dev Biol. 2012 Feb 25;365(1):267–276. doi: 10.1016/j.ydbio.2012.02.030

Fig. 3.

Fig. 3

Nemo potentiates Eya-mediated induction of Dac expression. (A–D) Dac (red), Eya (blue) and Nmo-GFP (green) expression in third-instar larval wing disks. In this and all other figures, wing disks are oriented dorsal up, anterior left. Eya and Nmo transgenes were driven using Dpp-GAL4. (A) Endogenous Dac expression. (B) Eya induces strong ectopic Dac expression in the dorsal hinge region (B”, arrow), and weaker expression in the wing disk pouch (B”, arrowhead). (C) Coexpression of Nmo increases Dac levels, particularly in the pouch and ventral region (C”’, arrowheads). (D) Coexpression of kinase-inactive Nmo (NmoK69M) decreases Eya-mediated Dac expression in the wing disk pouch (D”’, arrowhead, compare to B”) but not in the hinge region (D”, arrow).