Figure 1.

Decreased expression of the miR-17∼92 cluster in human idiopathic pulmonary fibrosis. (A) Expression of each microRNA (miRNA) from the miR-17∼92 cluster was determined by quantitative real-time polymerase chain reaction from control (n = 10), >80% FVC (n = 7), 50–80% FVC (n = 8), and <50% FVC (n = 9) lung tissue samples. Data were normalized to miR-191. Relative copy number (RCN, 2^−dCT × 100) was determined. Data are expressed as the average RCN ± SD, *P < 0.018 compared with control tissue. Comparison of the mild disease (80% FVC) with control tissue for miR-19b and -92, P = 0.1325 and 0.1320, respectively. (B) In situ hybridization was performed using LNA-modified DNA probes for let-7c (positive control) and miR-19b and -20a at a magnification of ×400. Scrambled probes were used as a negative control. The arrows denote positively stained cells (dark blue/purple). Shown are representative images (n = 3 per group).