Figure 3. Multiple alignment of VP2 encoding sequences (nucleotides 228 to 1755).
CLUSTAL W was used to align vp2 nucleotide sequences from wild carnivore parvoviruses characterized during this study and from parvoviruses representatives of each virus type. FPLV, on the top line, is represented by the vp2 sequence from a lion isolate (EF418569). Sequences 22124-7 (JF422105), 41220-1 and 22124-8 (JF422106) were obtained from E. mongooses, while sequences 41219-1, 41219-2 and 41219-3 refer to red fox specimens and sequence 41524 to the stone marten isolate. Other virus types are represented by strains M23255 (CPV-2), DQ340410 (CPV-2a), DQ340422 (newCPV-2a), AB054222 (newCPV-2a-Asp300), AF306450 (CPV-2b), AB054221 (newCPV-2b), AB054224 (newCPV-2b-Asp300), AF306449 (CPV2b-Pro265) and FJ005240 (CPV-2c). Nucleotides which are identical to those in FPLV are represented by dots, while those that differ are indicated. Dashes represent non determined nucleotides. The position of nucleotides within vp2 is indicated above FPLV reference sequence. Triplets corresponding to characteristic amino acid positions are shaded and their position is indicated below each triplet. Triplets harboring nucleotide variations between feline and canine strains that encode the same amino acid are also shaded and referred as synonymous.
