Table 2. Methods, tissues and genomic regions used for virus detection.

Viruses	Nucleic acid	Kit used for amplification	Type ofPCR	Genomic region Targeted	Size of the amplicon	Tissue	Reference
CPV/FPLV	DNA	Fast start Master Mix, Roche	real-time	vp2 gene	93 bp	small intestine lymph nodes	[46]
FCoV/CCoV	RNA	One-step RT-PCR, Qiagen	real-time	7b gene	102 bp	small intestine lymph nodes	[41]
CDV	RNA	One-step RT-PCR, Qiagen	real-time	N gene	161 bp	lungs	in house (not published)
ADV	DNA	Fast start Master Mix, Roche	real-time	gB gene	94 bp	lungs	[42]
CAV-1	DNA	High Fidelity Master Mix, Roche	conventional	E3 gene	508 bp	liver	[43]
CAV-2	DNA	High Fidelity Master Mix, Roche	conventional	E3 gene	1030 bp	lungs	[43]
FHV	DNA	High Fidelity Master Mix, Roche	real-time	TK gene	56 bp	lungs	[44]
Influenza A	RNA	One-step RT-PCR, Qiagen	real-time	Matrix gene	100 bp	lungs	[45]