Table 3. Primer sequences and their positions in the genome of parvovirus.

Primer	Nucleotide sequence (5′–3′)	Positiona	Sense	Specificity	Ref.	Purpose of use
CPV-For	AAACAGGAATTAACTATACTAATATATTTA	4101–4130	+	FPLV&CPVm	[46]	Viral survey
CPV-Rev	AAATTTGACCATTTGGATAAACT	4138–4167	−	FPLV &CPV
CPV-Probe	FAM-TGGTCCTTTAACTGCATTAAATAATGTACC-TAMRA	4171–4193	+	FPLV &CPV
CPV-1F	ACCAGATCATCCATCAACATC	2653–2673	+	FPLV &CPV	[51]	vp2 gene amplification
CPV-1R	CAATTAGTTGCCAATCTCCTG	3153–3173	−	FPLV &CPV
CPV-2F	AAATTGTAACACCTTGGTCATTG	3096–3118	+	FPLV &CPV
CPV-2R	AAATGGTGGTAAGCCCAATG	3636–3655	−	FPLV &CPV
CPV-3F	ACAGGTGATGAATTTGCTACAG	3554–3575	+	FPLV &CPV
CPV-3R	TTACAGGAAGGTTAAAGTTAAT	4037–4058	−	FPLV &CPV
CPV-4F	CAACAGGAGAAACACCTGAG	3954–3974	+	FPLV&CPVm
CPV-4R	TCTTCTATTTCTTACAGTTATTG	4718–4740	−	FPLV &CPV
555-For	CAGGAAGATATCCAGAAGGA	4002–4021	+	FPLV &CPV	[52]	vp2 gene amplification
555-Rev	GGTGCTAGTTGATATGTAATAAACA	4561–4585	−	FPLV &CPV
P1	ATGAGTGATGGAGCAGTTC	2786–2804	+	FPLV &CPV	[12],[50]	vp2 gene amplification
PR	TTTCTAGGTGCTAGTTGAG	4512–4530	−	FPLV &CPV

^aposition in the complete genome of strain CPV-N (NC001539). ^mmismatch in some isolates.