Figure 2. Expression of Nnat isoforms in response to fasting versus ad-libitum feeding.

A) Hypothalamic Nnat-α and -β showed a non-significant reduction in response to overnight fasting (n = 10) when compared to ad-libitum fed counterparts (n = 10); B) but a significant reduction after 24-h fasting (n = 10) compared to feeding (n = 11), equivalent for both isoforms (**Nnat-α P = 0.005, **Nnat-β P = 0.007); C–D) Nnat isoforms did not show a consistent or significant change after overnight fasting in the stomach or duodenum (n = 10 fasted, n = 10 ad-libitum fed in both tissues); key – AU = arbitrary units where Nnat expression was standardised using ubiquitin (Ubc) as a reference gene.