Figure 3. Hypothalamic and brainstem Nnat expression after modified gastric bypass versus sham surgery.

A) Nnat-β showed a significant reduction in the hypothalamus (**P = 0.003) after modified gastric bypass (n = 8) compared to sham surgery (n = 7) whilst Nnat-α did not reduce significantly, consistent with a bypass-specific effect on Nnat-β expression; B) expression of Nnat-α and Nnat-β did not differ in the brainstem after modified gastric bypass (n = 8) compared to sham surgery (n = 7); key – GBP = modified gastric bypass surgery, Sham = sham surgery, AU = arbitrary units where Nnat expression was standardised using an endogenous reference gene (ubiquitin (Ubc) for hypothalamus, hypoxanthine guanine phosphoriboribosyl transferase (Hprt) for brainstem).