Figure 5. P. aeruginosa flagella activate TLR5-NF-κB signaling in airway epithelial cells.

A) Purified P. aeruginosa flagellin was applied to 16HBE cells for 4 h before RNA was extracted and gene levels quantitated by qRT-PCR. *P<0.05, **P<0.01, ***P<0.001, TLR5 inhibitor is compared to flagella alone while dynasore is compared to the DMSO alone control (students t test). N = 3 and is representative of two independent experiments. Dyn-dynasore. B) Primary murine nasal epithelial cells were stimulated with purified flagella or P. aeruginosa (Pa) for 4 h before levels of Ifnb were quantitated by qRT-PCR.