Skip to main content
. 2013 Mar 20;8(3):e60016. doi: 10.1371/journal.pone.0060016

Figure 2. RA-induced epithelial differentiation correlates with β-catenin membrane localization.

Figure 2

(A) Representative β-catenin (green) immunocytochemistry and Hoechst (blue) staining of H9 hESCs cultured without or with RA in UM for 4 days. (B) Quantitative polymerase chain reaction (PCR) of cDNA harvested from H9 cells cultured in UM with or without RA for 4 days. Expression of Wnt signaling target genes T, FZD7 and MYC was significantly reduced upon RA addition; *p<0.05, UM versus RA. Error bars represent SEM of three biological replicates.