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. 2013 Mar 20;8(3):e58301. doi: 10.1371/journal.pone.0058301

Figure 3. Sxl and Bam associate with Mei-P26.

Figure 3

(A) Sxl immunoprecipitates with Mei-P26 in ovarian extracts from newly eclosed females. (B) Heat-shock induced Bam::HA and Sxl co-immunoprecipitate with Mei-P26. The levels of Sxl that associate with Mei-P26 do not appear to change in the presence of Bam::HA. (C) Extracts from whole ovaries were fractionated and the resulting eluents were probed for the presence of various proteins using western blot analysis. Mei-P26, GFP-tagged Bgcn, Sxl and HA-Bam were observed over a range of fractions but co-peaked in fraction 30. The control proteins Ter94 and Actin peaked in fractions 34 and 37 respectively.