Table 1. Statistics for the sequences retrieved from the 16 S rDNA over the V5–V6 and V8 variable regions.
A. Total sequences | ||
V5–V6 | V8 | |
Total sequences | 106,366 | 41,514 |
Classified Bacteria | 79,532 | 37,671 |
Unclassified Bacteria | 26,834 | 3,843 |
B. Total sequences by sample (plastid sequences removed | ||
V5V6 | V8 | |
Fall blade 1 | 8154 | 2613 |
Fall blade 2 | 3516 | 3864 |
Fall blade 3 | 10,296 | 2438 |
Fall blade 4 | 8405 | 2936 |
Fall blade 5 | 7393 | 2863 |
Winter blade 1 | 7875 | 4362 |
Winter blade 2 | 2658 | 3771 |
Winter blade 3 | 2581 | 4279 |
Winter blade 4 | 18129 | 4874 |
Winter blade 5 | 22790 | 5397 |
Lab blade 1 | 8210 | 2174 |
Lab blade 2 | 6359 | 1943 |
Total | 106,366 | 41,514 |
Total sequences are those recovered after quality curation (denoising, trimming, chimera removal, and removal of P. umbilicalis chloroplast sequences).