Table 3. Core and abundant OTUs found in the Porphyra umbilicalis microbiome identified using V8 (Taxonomic level 2 = phylum, 3 = class, 4 = order, 5 = family, 6 = genus).
A. Core Microbiomea | |||
Tax level | Taxon | No. of OTUs | OTU code number |
2 | Bacteroidetes | 7 | |
3 | Sphingobacteria | 4 | |
5 | Saprospiraceae | 4 | 1982(F)*, 2434(E)*, 2521(W)**, 2525(E)* |
3 | Flavobacteria | 2 | |
5 | Flavobacteriaceae | 2 | 2410(F)*, 2427(W)* |
3 | Unclassified | 1 | 2387(W) |
2 | Chloroflexi | 1 | 2493(E)* |
2 | Proteobacteria | 5 | |
3 | Gammaproteobacteria | 1 | |
6 | Granulosicoccus | 1 | 2408(W)* |
3 | Alphaproteobacteria | 4 | |
5 | Hyphomonadaceae | 1 | 2494(W)* L |
5 | Rhodobacteraceae | 1 | 2225(E)* L |
5 | Unclassified | 2 | 1933(E), 2219(W)* |
B. Lab: Most abundant OTUsb | |||
Tax level | Taxon | No. of OTUs | OTU code number |
2 | Bacteroidetes | 3 | |
3 | Sphingobacteria | 1 | |
5 | Saprospiraceae | 1 | 2521 |
3 | Flavobacteria | 2 | |
5 | Flavobacteriaceae | 2 | 2367, 2427 |
2 | Planctomycetes | 1 | 1352 |
2 | Proteobacteria | 4 | |
3 | Gammaproteobacteria | 1 | |
5 | Alteromonadaceae | 1 | 2182 |
3 | Alphaproteobacteria | 1 | |
5 | Rhodobacteraceae | 1 | 2225 |
5 | Sphingomonadaceae | 1 | |
6 | Sphingopyxis | 1 | 1938 |
C. Field: Most abundant OTUsc | |||
Tax level | Taxon | No. of OTUs | OTU code number |
2 | Bacteroidetes | 9 | |
3 | Sphingobacteria | 9 | |
4 | Sphingobacteriales | 3 | 1875, 2381, 2383 |
5 | Saprospiraceae | 4 | 2434, 2497, 2521, 2525 |
6 | Lewinella | 2 | 2302, 2475 |
3 | Flavobacteria | 1 | |
5 | Flavobacteriaceae | 1 | 2410 |
3 | Unclassified | 1 | 2435 |
2 | Proteobacteria | 1 | |
3 | Alphaproteobacteria | 1 | 2514 |
2 | Unclassified (Bacteria) | 3 | 2489, 2500, 2524 |
13 OTUs (0.03 distance) found across fall, winter & lab groups.
present at≥1% of all sequences on at least one blade (n = 12)
L≥1% on a lab blade
present at ≥1% on all 12 blades
OTUs (0.03 distance) with sequence abundance greater than 1% on both lab blades
OTUs (0.03 distance) with sequence abundance greater than 1% on at least 3 of the 10 winter (n = 5) and fall (n = 5) blades; underlined OTUs present on at least 5 of 10 blades; OTU 2521 present on all.
Designations of F = Fall, E = Evenly distributed between fall and winter, W = Winter refer to a ≥3× skew in sequence abundance toward that group.