Table 4. Core and abundant OTUs found in the Porphyra umbilicalis microbiome identified using V5V6 (Taxonomic level 2 = phylum, 3 = class, 4 = order, 5 = family, 6 = genus).
A. Core Microbiomea | |||
Tax level | Taxon | No. of OTUs | OTU code number |
2 | Bacteroidetes | 9 | |
3 | Sphingobacteria | 2 | |
4 | Sphingobacteriales | 2 | 4509(W)*, 4580(W)* |
3 | Flavobacteria | 6 | |
5 | Flavobacteriaceae | 6 | 3834(E), 3913(E), 3944(F), 3969(E), 4507(F)*, 4510(W)* L |
2 | Chloroflexi | 1 | 4599(W)* |
2 | Planctomycetes | 2 | |
3 | Phycisphaerae | 1 | |
5 | Phycisphaeraceae | 1 | |
6 | Phycisphaera | 1 | 3875(W) |
3 | Planctomycetacia | 1 | |
5 | Planctomycetaceae | 1 | 3994(F)* |
2 | Proteobacteria | 1 | |
3 | Alphaproteobacteria | 1 | |
5 | Rhodobacteraceae | 1 | 4349(W)* L |
2 | Unclassified(Bacteria) | 6 | 2742(E), 4252(F)*, 4368(E), 4392(F)*, 4453(W),4491(E)* |
B. Lab: Most abundant OTUsb | |||
Tax level | Taxon | No. of OTUs | OTU code number |
2 | Bacteroidetes | 1 | |
3 | Flavobacteria | 1 | |
5 | Flavobacteriaceae | 1 | 4510 |
2 | Proteobacteria | 1 | |
3 | Alphaproteobacteria | 1 | |
5 | Rhodobacteraceae | 1 | 4349 |
2 | Unclassified (Bacteria) | 1 | 2443 |
C. Field: Most abundant OTUsc | |||
Tax level | Taxon | No. of OTUs | OTU code number |
2 | Bacteroidetes | 3 | |
3 | Sphingobacteria | 2 | |
4 | Sphingobacteriales | 2 | 4509, 4580 |
3 | Flavobacteria | 1 | |
5 | Flavobacteriaceae | 1 | 4507 |
2 | Chloroflexi | 1 | 4599 |
2 | Unclassified (Bacteria) | 2 | 4392, 4491 |
19 OTUs (0.03 distance) shared between Fall, Winter & Lab Groups
present at ≥1% of all sequences on at least one blade (n = 12)
L ≥1% on a lab blade
OTUs (0.03 distance) with sequence abundance greater than 1% on both lab blades
OTUs (0.03 distance) with sequence abundance greater than 1% on at least 3 of the 10 winter (n = 5) and fall (n = 5) blades
Designations of F = Fall, E = Evenly distributed between fall and winter, W = Winter refer to a ≥3× skew in sequence abundance toward that group.