Figure 4. CHIR99021 enhances DN3 development in the absence of preTCR signalling.

Rag-2^−/− DN3 cells were cultured on OP9-DL1 or OP9 cells in the absence or presence of CHIR99021 (3 or 10 µM). After 48 and 72 hours cells were harvested and analyzed for the percentage of CD4⁺CD8⁺, CD4⁺CD8⁻, CD4⁻CD8⁺ and CD4⁻CD8⁻ cells (a) and at 72 hours the total number of cells harvested relative to input were determined (b). The graph shows the mean and standard deviation of three biological replicates conducted on three different days. The p values were determined using the paired Student's t test (*  =  p<0.05 and ** =  p<0.01).