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. 2013 Mar 20;8(3):e59889. doi: 10.1371/journal.pone.0059889

Figure 4. Virus composition in ferrets inoculated with H5N1-pH1N1 reassortant virus during the course of infection.

Figure 4

Two ferrets were inoculated intranasally with 107.3 TCID50 of the MDCKP1 H5N1-pH1N1 reassortant virus mixture. At day 1, 3, 5 and 7 dpi throat swabs were collected from both ferrets (F1 and F2). In these throat samples, as well as the MDCKP1 inoculum, the ratio of the H5N1 (red) and pH1N1 (blue) gene segments (PB2, PB1, PA, NP, NA, M and NS) was determined by pyrosequencing. ND: no detection of viral gene segments by PCR.