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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Biochem Pharmacol. 2013 Feb 8;85(8):1195–1202. doi: 10.1016/j.bcp.2013.01.031

Fig. 1. Kinetics of PTI metabolism by mouse, rat and human liver microsomes.

Fig. 1

A: Time courses of the levels of PTI and IND in PTI-treated liver microsomes. PTI (75 µM) was incubated with mouse, rat and human liver microsomes (protein concentration 0.5 mg/ml) at 37°C for up to 3 h. PTI and its metabolites were extracted at the designated time points and assayed using HPLC. Inset, the structure of PTI. B: Time course of metabolite formation in PTI-treated liver microsomes. C: Comparison of metabolite formation in PTI-treated HLMs in the presence (+) versus absence (−) of NADPH, 20 min after the initiation of the reaction.