Figure 5.
mRNA expression of genes involved in bile salt and organic anion homeostasis in larval and adult lampreys. Real-time RT-PCR was performed using cDNA from liver (A), kidney (B), the proximal half of the intestine (C), the distal half of the intestine (D), and the gills (E). Because there was no significant difference between males (n = 4) and females (n = 4), and because we were not able to differentiate the gender of lamprey larvae (n = 5), gene expression data from adult males and females were combined for analysis and presentation. Data of larvae and adults were normalized to lamprey β-actin, and presented as means ± SD by setting larval expression as 1. * p < 0.05 versus larvae; # p < 0.01 versus larvae.