Figure 1.

Treatment with platelet-derived growth factor (PDGF) induces the spatial translocation of Raf-1 in human airway smooth muscle (HASM) cells. HASM cells were stimulated with PDGF (10 ng/ml) for 10 minutes or left unstimulated. The cellular localization of Raf-1 was evaluated by immunofluorescent microscopy. (A) Representative images illustrating the spatial redistribution of Raf-1 in response to activation with PDGF. The inserts are the 1.5× magnification of the selected area. Dashed arrows indicate a single line scan to quantify the fluorescent signal for each cell. C = control. (B) Protein distribution in cells is expressed as ratio of pixel intensity at the cell periphery/pixel intensity at cell interior. Data are means ± SE. *Significantly different between unstimulated cells and stimulated cells (P < 0.05; n = 15).