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. 2013 Feb;48(2):172–178. doi: 10.1165/rcmb.2012-0315OC

Figure 5.

Figure 5.

Lentivirus-mediated knockdown (KD) and rescue of Abl in HASM cells. Cells were coinfected with lentiviruses encoding Abl shRNA and viruses encoding inducible Abl for 3 days. Blots of control, KD, and rescue cells were probed with the use of antibodies against Abl and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). (A) Representative immunoblots illustrating the expression of Abl in KD cells and rescue cells. (B) Ratios of Abl/GAPDH protein in Abl KD cells and rescue cells were normalized to ratios obtained from control cells. Values are means ± SE (n = 4). *Significantly lower Abl/GAPDH ratios in KD cells compared with control cells and rescue cells (P < 0.05). Control, cells were uninfected; KD (knockdown), infected cells were not treated with doxycyclin; Rescue, infected cells were treated with doxycyclin (0.5 μg/ml, 24 h).