Table 4.

Criteria to optimize success of acquisition of high quality tumor tissue biopsies

Clinical factor	Tumor factor*
No limit up to BMI 41.5 kg/m2	Minimum size 1.0 cm longest diameter on radiographic imaging22
Tumor location limited by safety, e.g. proximity to vessels and bowel wall	Less than 25% of tissue necrosis preferred.
No effect of recent or ongoing bevacizumab exposure	At least 50% of tumor cells in tissue obtained is preferred unless it is recognized that signal may reflect stroma and tumor
No limit to number or kinds of prior treatment19

^*Usable for IHC and/or RPPA