TABLE 2.

THE DELAYED ADDITION OF FGF-2 STILL ATTENUATES TGF-STIMULATED RESPONSES IN THE PRESENCE OF THE TGF-β–RECEPTOR INHIBITOR, SB431542

Treatment	Control	TGF-β	SB431542 (t = 23 h) + TGF-β	FGF-2 (t = 24 h) + SB431542 (t = 23 h) + TGF-β
SM22	9 ± 5	100	57 ± 8	36 ± 8*
Calponin	16 ± 12	100	65 ± 11	14 ± 6*

Definition of abbreviations: FGF-2, basic fibroblast growth factor; SM22, transgelin; TGF-β, transforming growth factor–β.

Relative levels of contractile protein mRNA (expressed as a percentage of TGF-β) in ASM cells incubated with TGF-β (100 pM) for 48 hours. SB431542 (10 μM) and FGF-2 (300 pM) were added 24 hours (t = 24 h) and 23 hours (t = 23 h) after TGF-β, respectively.

^*P < 0.05, versus SB431542 + TGF-β (n = 4).