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. 2013 Mar;48(3):337–345. doi: 10.1165/rcmb.2012-0393OC

Figure 3.

Figure 3.

In vivo siRNA silencing of airway DUOX1 in mice. (A) Study design illustrates timing of oropharyngeal (o.p.) siRNA instillation, naphthalene injection, and harvesting of lung tissues for analysis. (B) Analysis of DUOX1 mRNA expression in lung tissues at 2 days after siRNA instillation. *P < 0.05, compared with NS-siRNA (n = 6). Lung tissue was harvested 2 days after siRNA instillation and naphthalene injection (2 DPN) or control injection with corn oil (Ctl) for the analysis of DUOX protein expression according to Western blot analysis of whole-lung lysates (C) or by immunofluorescence analysis of lung-tissue sections (D), using an α-DUOX antibody (provided by Dr. F. Miot). i.p., intraperitoneal.