Figure 4.

In vivo DUOX1 silencing attenuates epithelial regeneration after naphthalene-induced injury. (A) Representative immunofluorescence images of lung sections obtained after 0, 2, 7, and 14 days after naphthalene injection (DPN), after staining for β-tubulin IV⁺ ciliated cells (green) and club cell secretory protein–positive (CCSP⁺) club cells (red). (B) Quantification of numbers of CCSP⁺ (club) cells per 100 μm using Metamorph software (Molecular Devices, Sunnyvale, CA), based on at least five airway sections per mouse. (C) Similar quantification of tubulin IV⁺ (ciliated) cells. Data represent the means ± SEs from 4–5 mice. *P < 0.05.