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. 2013 Mar 20;8(3):e59194. doi: 10.1371/journal.pone.0059194

Figure 3. IL-27 inhibits HIV-1 replication in DCs in a dose-dependent manner.

Figure 3

A: iDCs and mDCs were infected with HIV-1Ba-L, and HIV replication was measured by the HIV-1 p24 antigen capture kit. These experiments used DCs from three donors. B: HIV-infected iDCs were cultured in the presence of different amounts of IL-27 for 14 days and HIV-1 replication was monitored by the HIV-1 p24 antigen capture kit. C: iDCs were pre-treated with either media alone (iDCs) or 100 ng/ml IL-27 for 48 hours (PreIL-27-iDCs) followed by infection with HIV-1Ba-L. The HIV-1 infected iDCs were cultured for 14 days without IL-27 as described in the Materials and Methods and then HIV-1 replication was determined using the HIV-1 p24 antigen captures kit. The data shows a representative result from two independent donors. D: HIV-infected iDCs were cultured in the presence of 100 ng/ml of each of the IL-12 family of cytokines (IL-12, IL-23, IL-27 and IL-35) for 14 days and anti-viral effect of each cytokine was determined using the HIV p24 antigen capture kit. The results show the combined results of three independent donors and depict % of HIV-1 replication compared to untreated cells (control) and SE.